amj [2019-07-23 09:43:43]

lipid II flippase, alternative to MurJ

Locus: BSU_04230

Isoelectric point: 10.60

Molecular weight: 29.30 kDa

Protein length: 269 aa

Gene length: 810 bp

Function: export of lipid II

Product: lipid II flippase

Essential: no

Synonyms: ydaH

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Groups of genes → Membrane proteins

Gene

Coordinates: 474,731 475,540

Phenotypes of a mutant

• an amj murJ double mutant is not viable PubMed

The protein

Protein family

• Amj family (single member, according to UniProt)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: amj
  Description: PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon

  Regulation

  • induced in the stationary phase due to the internal production of toxic peptides (SdpC, SkfA) PubMed

Biological materials

Mutant

• MGNA-C089 (ydaH::erm), available at the NBRP B. subtilis, Japan
• BKE04230 (amj::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTATTCTTCCTCCAAT, downstream forward: _UP4_TAATAAGAAAGGCTGGATCA
• BKK04230 (amj::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTATTCTTCCTCCAAT, downstream forward: _UP4_TAATAAGAAAGGCTGGATCA

References

Reviews

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Original Publications

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