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amj [2019-07-23 09:43:43]
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amj [2019-07-23 09:43:43]

lipid II flippase, alternative to [protein|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|MurJ]
locus
BSU_04230
pI
10.60
mw
29.30 kDa
protein length
269 aa Sequence Blast
gene length
810 bp Sequence Blast
function
export of lipid II
product
lipid II flippase
essential
no
synonyms
ydaH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    474,731 475,540

    Phenotypes of a mutant

  • an ''[gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj] [gene|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]'' double mutant is not viable [Pubmed|25918422]

    • The protein

    Protein family

  • Amj family (single member, according to UniProt)

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj]
    description
    [Pubmed|17434969]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|17434969], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

    • regulation

  • induced in the stationary phase due to the internal production of toxic peptides ([SW|SdpC], [SW|SkfA]) [Pubmed|26364265]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C089 (ydaH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2087 NBRP B. subtilis, Japan]
  • BKE04230 ([gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTATTCTTCCTCCAAT, downstream forward: _UP4_TAATAAGAAAGGCTGGATCA
  • BKK04230 ([gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTATTCTTCCTCCAAT, downstream forward: _UP4_TAATAAGAAAGGCTGGATCA

    • References

    Reviews

  • 26679002,28975672

    • Original Publications

  • 17434969,25918422,17434969,26364265